Abstract

AbstractProcess parameters were optimized for enzyme (textile‐grade enzymes) hydrolysis of Azolla, water hyacinth, and algae biomass for recovery of maximum sugar to be used as feed for biohydrogen production by Enterobacter cloacae strain DT‐1. Under optimum temperature and pH, the hydrogen yield in mole per mole of sugar from enzyme‐hydrolyzed water hyacinth biomass, Azolla biomass, and Spirulina and Scenedesmus biomass was determined with regard to cellulase, hemicellulase, amylase, pectinase, and xylanase. The maximum hydrogen yield was observed with hemicellulase‐treated Spirulina, Azolla, and Scenedesmus biomass.

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