BioHJzyme-Armed Hydrogel for Infectious Wound Monitoring Through Enzyme-Mimetic Antibacterial Activities and Macrophage Regulation

Abstract

The pathogenic colonization and the triggered disorder of macrophage phenotype evoked by pathogenic infection in wound inevitably leads to the stagnant wound regeneration. Herein, we devise ZnFe2O4/MXene bio-heterojunction enzyme (BioHJzyme)-armed hydrogel for infectious wound monitoring, which shows in-built macrophage re-rousing capability and enzyme-mimetic antibacterial (EMA) activity, including glutathione peroxidase (GPx) -mimetic activity and peroxidase (POD)-mimetic activity with a turnover number of 0.30 s−1. The anti-pathogenic efficiency can be further enhanced upon near infrared laser (NIR) irradiation with a turnover number of 1.48 s−1. On top of that, the in vivo experimental results demonstrate that the BioHJzyme-armed hydrogel possess overarching antibacterial activities and anti-inflammation through regulating macrophage phenotype, coupled with enriched collagen deposition and angiogenesis. As envisaged, this work provides a tactic in the realm of infectious wound monitoring via enzyme-mimetic antibacterial activities and macrophage re-rousing.