Biogenic synthesis of copper nanoparticle using Impatiens chinensis L: insights into antimicrobial, antioxidant and anticancer activity

Abstract

Impatiens chinensis plant is a various medicinal properties and use in the field of biomedicine in several decades. In present of the study, successfully green synthesized copper nanoparticles (CuNPs) using the leaf extract of I. chinensis. The synthesized CuNPs were thoroughly characterized using various techniques such as UV, FTIR, XRD, and SEM with EDAX, TEM, particle size and Zeta potential analysis. The UV–Vis analysis confirmed the successful synthesis of CuNPs, as indicated by absorbance peak at 313 nm. FTIR spectra revealed the different functional groups and molecular bonding interactions of the CuNPs, while XRD analysis unveiled the crystalline nature of the NPs. SEM imaging confirmed the spherical structure of the CuNPs and EDAX confirmed the Copper (Z), Oxygen (O) and CuL (Cl) elements. TEM imaging confirming the spherical shape and identifying the SAED pattern. Moreover, the particle size analysis the size of 63.1 nm identified and the zeta potential range of -27.1 mv confirmed their stability. To evaluate the antibacterial activity of different pathogenic bacteria using various zone of inhibitions. CuNPs antioxidant activity, as evidenced by DPPH (53.20%), ABTS (52.37%) and Hydroxyl scavenging assays (51.02%). The evaluate CuNPs for their potential anti-cancer properties in MCF-7 cell lines, showing (65.34%) inhibition. Overall, the findings of this study green-synthesized CuNPs as multifunctional agents with antibacterial, antioxidant and anticancer properties. These nanoparticles hold potential for biomedical applications, providing sustainable solutions for a range of health related challenges.