Biogenic Synthesis and Characterization of Antioxidant and Antimicrobial Silver Nanoparticles Using Flower Extract of Couroupita guianensis Aubl.

Reetika Singh,Bechan Sharma,Francesco Tavanti,Christophe Hano

doi:10.3390/ma14226854

Abstract

Couroupita guianensis Aubl. is an important medicinal tree. This tree is rich in various phytochemicals, and is therefore used as a potent antioxidant and antibacterial agent. This plant is also used for the treatment of various diseases. Here, we have improved its medicinal usage with the biosynthesis of silver nanoparticles (AgNPs) using Couroupita guianensis Aubl. flower extract as a reducing and capping agent. The biosynthesis of the AgNPs reaction was carried out using 1 mM of silver nitrate and flower extract. The effect of the temperature on the biosynthesis of AgNPs was premeditated by room temperature (25 °C) and 60 °C. The continuous stirring of the reaction mixture at room temperature for approximately one hour resulted in the successful formation of AgNPs. A development of a yellowish brown color confirmed the formation of AgNPs. The efficacious development of AgNPs was confirmed by the characteristic peaks of UV–Vis, X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy spectra. The biosynthesized AgNPs exhibited significant free radical scavenging activity through a DPPH antioxidant assay. These AgNPs also showed potent antibacterial activity against many pathogenic bacterial species. The results of molecular dynamics simulations also proved the average size of NPs and antibacterial potential of the flower extract. The observations clearly recommended that the green biosynthesized AgNPs can serve as effective antioxidants and antibacterial agents over the plant extract.

Highlights

Nanotechnology is a well-recognized and exponentially growing field with potential applications in the medical and non-medical field [1,2,3,4,5]
The controlled biosynthesis of metal nanoparticles offers a significant effect by providing a definite size and morphology of NPs, which is different from results obtained by physicochemical methods
AgNO33 began changing its color from colorless to yellowish brown in approximately 10–15 min, and, the solution turned from a light brown to dark brown color in approximately approximately 40–45 min min at at room room temperature

Summary

Introduction

Nanotechnology is a well-recognized and exponentially growing field with potential applications in the medical and non-medical field [1,2,3,4,5]. Nanotechnology is a growing field that makes an impact in all provinces of human life [7], dealing with the synthesis, development and applications of a variety of nanoparticles (NPs). The controlled biosynthesis of metal nanoparticles offers a significant effect by providing a definite size and morphology of NPs, which is different from results obtained by physicochemical methods. These NPs may be defined as particles whose sizes range from 1–100 nm.

Results

Discussion

Conclusion