Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity.

B Gowramma,D Muralidhara Rao,U Keerthi,Mokula Rafi

doi:10.1007/s13205-014-0210-4

B Gowramma, D Muralidhara Rao + Show 2 more

Open Access

https://doi.org/10.1007/s13205-014-0210-4

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Journal: 3 Biotech	Publication Date: Apr 8, 2014
Citations: 61	License type: CC BY 4.0

Affiliation: Sri Krishnadevaraya University

Abstract

In the present study, synthesis, characterization, and the antibacterial activity of silver nanoparticles from native isolate of Corynebacterium glutamicum has been reported. Silver nanoparticles were synthesized by challenging the dried biomass of C. glutamicum with aqueous diamine silver ([Ag (NH3)2]+) containing 1 mM AgNO3. Synthesized silver nanoparticles (AgNPs) were characterized by ultraviolet–visible spectroscopy and energy-dispersive X-ray (EDX) spectroscopy analysis. Morphological study of silver nanoparticles was carried out using transmission electron microscopy (TEM) and scanning electron microscope (SEM). The spherical morphology of silver nanoparticles was confirmed from SEM image. The TEM image showed the average particle size of silver nanoparticles was about 15 nm. Silver nanoparticles synthesized from C. glutamicum were found to have enhanced antimicrobial activity against selected pathogenic strains. Silver nanoparticles from pure strains of Corynebacterium species was done by many investigators, but as per the present literature, this is the first report on the production of silver nanoparticles using a native strain of Corynebacterium.

Highlights

Nanotechnology is referring to the ability for designing, characterization, production and application of structures, devices and systems by controlling shape and size at the nano scale (Mansoori et al 2007) and it is a promising emerging industry, which is bringing us exiting new products
Synthesis of nanoparticles have been demonstrated from some microbes, among them the bacterial species used have included Escherichia coli (Natarajan et al 2010), Bacillus cereus (Sunkar and Nachiyar 2012), Corynebacterium sp. (Arun et al 2013), while yeast species have included MKY3 yeast strain (Kowshik and Ashataputre, 2003), Saccharomyces cerevisiae BU-MBT CY-1 (Selvakumar et al 2011), fungi included T. asperellum (Mukherjee et al 2008), Aspergillus clavatus (Verma et al 2010) Trichoderma Reesei (Vahabi et al 2011), Aspergillus terreus (Li et al 2012), algae Cyanobacteria (Sudha et al 2013) and lichen Parmotrema praesorediosum (Mie et al 2013) are able to absorb and accumulate metal and can be used in the reduction of environmental pollution and for the recovery of
The presence of nanoparticles in the medium was confirmed by the change in color from colorless to brown or deep yellow shown in Fig. 1b, where control showed no color formation in the culture when incubated for the same period and conditions (Fig. 1a)

Summary

Introduction

Nanotechnology is referring to the ability for designing, characterization, production and application of structures, devices and systems by controlling shape and size at the nano scale (Mansoori et al 2007) and it is a promising emerging industry, which is bringing us exiting new products. There is a growing need to use environmental friendly nanoparticles that do not produce toxic wastes in their synthesis protocol (Vahabi et al 2011). Many methods have been designed to synthesize nanoparticles and the most important aspects of nanotechnology rely on the synthesis of nanoparticles with well-defined sizes, shapes and controlled monodispersity (Pugazhenthiran et al 2009). Biotechnological route has emerged as a safe and alternative process in the synthesis of nanoparticles by employing ambient biological resources (Baker et al 2013). Studies have reported that the biological methods depict an inexpensive and ecofriendly route for synthesis of nanoparticles. Synthesis of nanoparticles have been demonstrated from some microbes, among them the bacterial species used have included Escherichia coli (Natarajan et al 2010), Bacillus cereus (Sunkar and Nachiyar 2012), Corynebacterium sp. (Arun et al 2013), while yeast species have included MKY3 yeast strain (Kowshik and Ashataputre, 2003), Saccharomyces cerevisiae BU-MBT CY-1 (Selvakumar et al 2011), fungi included T. asperellum (Mukherjee et al 2008), Aspergillus clavatus (Verma et al 2010) Trichoderma Reesei (Vahabi et al 2011), Aspergillus terreus (Li et al 2012), algae Cyanobacteria (Sudha et al 2013) and lichen Parmotrema praesorediosum (Mie et al 2013) are able to absorb and accumulate metal and can be used in the reduction of environmental pollution and for the recovery of

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Conclusion