Abstract
This study was conducted for the synthesis of silver nanoparticles by using olive leaves aqueous extract and evaluate its antibacterial activity against Pseudomonas aeruginosa in vitro. The synthesis and characterization of silver nanoparticles was confirmed by Ultra Violet Visible – spectrophotometer and Scanning Electron Microscopy. Well diffusion method was used to show the antibacterial action of silver nanoparticles against Pseudomonas aeruginosa in vitro in comparison with standard antibacterial silver sulfadiazine by using different concentrations of each agent ranged from 12.5-200 μg/ml. The results of this study showed it possible to produce silver nanoparticles in eco-friendly and easy process and UV-Visible absorption spectra of the silver nanoparticles revealed maximum absorbance at 420 and 430 nm. The Scanning Electron Microscopy analysis demonstrated the mean of the silver particles diameter was 26 nm. The antibacterial findings of the synthesized silver nanoparticles against Pseudomonas aeruginosa in vitro showed that the silver nanoparticles were more effective than silver sulfadiazine against Pseudomonas aeruginosa. It could be concluded that olive leaves extract can be used effectively in the production of silver nanoparticles and these synthesized nanoparticles had considerable antibacterial activity against Pseudomonas aeruginosa in vitro.
Highlights
When olive leaves extract was mixed with the AgNO3 solution at room temperature gave pale yellow color, after 5 min. of reaction time at 40°C the color of the solution altered from faint yellow to profound or deep yellow color at 40°C and 5 min. of reaction time
When the temperature of water bath increased to 60°C for 10 min. the color of blend altered to deep brown color, and the color of the mixture became grey-black at 60°C after 15 min. of reaction time (Fig. 1)
1: Color change during synthesis of silver nanoparticles a- Pale yellow color when olive leaves extract was mixed with the silver nitrate solution. b- Deep yellow color at 40°C after 5 min. of reaction time. c- Deep brown color at 60°C after 10 min. of reaction time. d- Grey-black color at 60°C after 15 min. of reaction time
Summary
This study was conducted for the synthesis of silver nanoparticles by using olive leaves aqueous extract and evaluating its antibacterial activity against Pseudomonas aeruginosa in vitro. Well diffusion method was used to show the antibacterial action of silver nanoparticles against Pseudomonas aeruginosa in vitro in comparison with standard antibacterial silver sulfadiazine by using different concentrations of each agent ranged from 12.5-200 μg/ml. The antibacterial findings of the synthesized silver nanoparticles against Pseudomonas aeruginosa in vitro showed that the silver nanoparticles were more effective than silver sulfadiazine against Pseudomonas aeruginosa. It could be concluded that olive leaves extract can be used effectively in the production of silver nanoparticles and these synthesized nanoparticles had considerable antibacterial activity against. The present study was conducted to synthesis the AgNPs biologically by olive leaves extract and evaluate its antibacterial activity against P. aeruginosa in vitro.
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