Abstract
Microbial technology offers a green alternative for the synthesis of value-added nanomaterials. In particular, fungal compounds can improve silver nanoparticle production, stabilizing colloidal nanoparticles. Based on a previous study by our group, silver nanoparticles obtained using the extracellular cell-free extracts of Phanerochaete chrysosporium (PchNPs) have shown antimicrobial and antibiofilm activity against Gram-negative bacteria. Moreover, nisin—a bacteriocin widely used as a natural food preservative—has recently gained much attention due its antimicrobial action against Gram-positive bacteria in biomedical applications. Therefore, the aim of this work was to conjugate biogenic silver nanoparticles (PchNPs) with nisin to obtain nanoconjugates (PchNPs@nis) with enhanced antimicrobial properties. Characterization assays were conducted to determine physicochemical properties of PchNPs@nis, and also their antibacterial and antibiofilm activities were studied. The formation of PchNPs@nis was confirmed by UV-Vis, TEM, and Raman spectroscopy analysis. Different PchNPs@nis nanobioconjugates showed diameter values in the range of 60–130 nm by DLS and surface charge values between −20 and −13 mV. Nisin showed an excellent affinity to PchNPs, with binding efficiencies higher than 75%. Stable synthesized PchNPs@nis nanobioconjugates were not only able to inhibit biofilm formation by S. aureus, but also showed inhibition of the planktonic cell growth of Staphyloccocus aureus and Escherichia coli, broadening the spectrum of action of the unconjugated antimicrobials against Gram-positive and Gram-negative bacteria. In conclusion, these results show the promising application of PchNPs@nis, prepared via green technology, as potential antimicrobial nanomaterials.
Highlights
In the last years, biological synthesis of nanomaterials using microorganisms has shown immense potential as an ecofriendly and cost-effective alternative to traditional methods
Which corresponds to the surface plasmon resonance (SPR)—confirmed the formation of the biogenic silver nanoparticles, PchNPs
Nanobioconjugates were produced by combining biogenic silver nanoparticles—
Summary
Biological synthesis of nanomaterials using microorganisms has shown immense potential as an ecofriendly and cost-effective alternative to traditional methods. Green approaches are based on the utilization of natural products (from microorganisms or plants) as reducing and stabilizing agents for the synthesis of biogenic metallic nanoparticles. These reactions are carried out under ambient conditions (room temperature and atmospheric pressure) and may reduce the toxicity of nanoparticles [1]. Our group has reported the development of an extracellular cell-free filtrate method for the biological synthesis of silver nanoparticles. Some of these biogenic nanoparticles showed antimicrobial activity against Gram-negative Escherichia coli and Salmonella enterica bacteria [7,8]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
