Abstract
A sustainable method for producing silver nanoparticles (AgNPs) using Vitis vinifera seed extract has been proposed. The formation of AgNPs was confirmed through spectroscopy, X-ray Diffraction (XRD), and electron microscopy. The UV-visible spectrophotometer revealed distinct absorbance peaks at 398.80 nm and 416.24 nm, signifying the presence of Surface Plasmon Resonance (SPR). XRD, quasi-elastic light scattering, and surface topography results exposed that the AgNPs were crystalline and had rod-like shapes. FTIR identified seed extract functional groups that reduced silver ions to AgNPs and acted as capping agents. The nanoparticles showed inhibition against gram-positive microorganisms like Staphylococcus pneumonia and Mycobacterium tuberculosis, surpassing their effectiveness against gram-negative microorganisms such as Pseudomonas fluorescens. This environmentally responsible approach not only offers a green synthesis method but also holds promise for combating specific pathogenic bacteria.
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