Abstract
Bacteria adherence and biofilm formation vary depending on the strain of microorganism as well as the substrate.Here, we evaluated the ability of different isolates of Staphylococcus aureus (S. aureus), isolated. from atopic dermatitis patients skin and stool to form a biofilm on different Lewis types saliva, including Le (a) , (b) ,(c) and Le a+b+ saliva. S. aureus isolates were cultured on tryptose soy broth.The bacteria were used to form a biofilm on 96- well flat bottom Microtitration plate that was coated with the respective, optimally diluted, processed saliva of Lewis blood groups (a),(b),(c) and Le a+b+.The intensity of biofilm formation was assessed by measuring the biofilm-associated optical density reading of bacterial bound dye. The results indicated a superior ability of biofilm formation on Lewis(a) saliva type.The highest binding gave a mean of 1.25 ± 0.39 compared to biofilm formation on non – coated wells that showed a mean of 0.23 ± 0.016(P=0.01, CL= 0.390 – 1.635). On the other hand, however, a variable degree of binding to Le(b) saliva was demonstrated for the isolates. The isolates didn’t demonstrate binding on Lea-b-(Lec) and Le a+b+ -(Led) saliva.
 We conclude that biofilm formation on Lewis types saliva of the atopic dermatitis bacterial isolates requires Le(a) in a ligand – receptor-based system for biofilm establishment.
Highlights
Biofilm is a microbial structure that causes numerous issues in restorative field, in nour- ishment industry and even to nature
Atopic dermatitis (AD) is an interminable fiery skin illness typically beginning in early youth with a revealed lifetime commonness of 15 – 30% in youngsters, while the com- paring figure for adults is 2– 10%. [6].Staphylococcus aureus is accounted for to rule in AD injuries and reports have uncovered the nearness of Staphylo- coccal biofilms
Lewis blood group molecules expressed in a variety of surfaces [11] and their role in bacterial colonization and biofilm formation is well de- fined [12] [13] [14].In this work it was tried to explore the role of Lewis blood groups determinants carried on salivary mucins on biofilm formation of S. aureus isolates from atopic dermatitis patients
Summary
Biofilm is a microbial structure that causes numerous issues in restorative field, in nour- ishment industry and even to nature. Along these lines, biofilm could cause numerous many public health problems. [6].Staphylococcus aureus is accounted for to rule in AD injuries and reports have uncovered the nearness of Staphylo- coccal biofilms. These infections add to irritation of the eczema [7]. Lewis blood group molecules expressed in a variety of surfaces [11] and their role in bacterial colonization and biofilm formation is well de- fined [12] [13] [14].In this work it was tried to explore the role of Lewis blood groups determinants carried on salivary mucins on biofilm formation of S. aureus isolates from atopic dermatitis patients
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
