Biofilm Formation and Attachment Factors Existence in Urinary Tract Infection Caused by Escherichia coli

Abstract

Introduction: Escherichia coli is a bacterium that is responsible for about 80% of all urinary tract infections. The formation of biofilm requires several cell surface factors such as flagella and motility, fimbriae, adhesins, autotransporter proteins, curli fimbriae, conjugative pilus, and exopolysaccharide production. Method: A total of 122 E. coli isolates were obtained from urine samples of patients with UTI. We examined the biofilm formation from the isolates in TSB, with supplemented 2% sucrose, using Congo Red Agar (CRA) method. The existence of the 2 studied genes (afa and fimA) in the isolates was evaluated using PCR assay. Result: E. coli isolates that produced and didn’t produce slime using CRA method were 81 (65.9%) and 36 (29.3%) respectively. Conclusion: E. coli bacteria isolates from UTI patients were able to form biofilmin CRA method and all E. coli causing UTI strains in human studied in this research positively produced biofilm, while some of the biofilm non-producing strains can be expressed in the 2 genes studied.