Biofilm Bacteria and Cholesteatoma on Chronic Suppurative Otitis Media

Abstract

Background: Chronic suppurative otitis media (CSOM) possibility to cause morbidity and mortality. Germinfections were a major factor in the long-standing inflammation of CSOM. Cholesteatoma is an epithelialcyst that containing keratin desquamation. The cholesteatoma present in CSOM patients is a kind of acquisitawith several theories that have been believed to be the basis of its formation.Objective: To explain the correlation between bacterial biofilms and cholesteatoma in CSOM.Method: We performed the prospective collection of tissue during mastoidectomy surgery from CSOMpatient. The tissue samples divided into two groups, cholesteatoma and non-cholesteatoma. All tissuesgroup processed for scanning electron microscopy (SEM) analysis. The SEM results will be categorizedinto positive and negative bacterial biofilms.Result: Statistical analysis using logistic regression test had p = 0.027 and risk ratio = 5.55. That meansthere was a significant correlation between bacterial biofilm and cholesteatoma in CSOM patients (p <0.05).CSOM with cholesteatoma has obtained the risk of bacterial biofilm by 5.55 times compared to CSOMwithout cholesteatoma.Conclusion: There was a correlation between bacterial biofilms and cholesteatoma in CSOM patients.A quarter of cholesteatoma showed positive biofilms. CSOM with cholesteatoma has a risk of positivebacterial biofilm.