Biofabrication of silver nanoparticles using diallyl sulphide (DAS) and comparison of cytotoxic potential of DAS-AgNPs and Kodaikanal Hill garlic in human cervical cancer

Abstract

Abstract Garlic (Allium sativum) exhibit well defined therapeutic properties due to presence of distinct bioactive compounds, specifically organosulphur compounds. Spectral analysis by UV–Vis and FT-IR confirmed interaction of different functional groups and free electrons with the electromagnetic waves showed presence of different bioactive compounds formulated silver nanoparticles (AgNPs) using diallyl sulphide (DAS) also done for aqueous garlic extract. Different assays performed for confirming the presence of antibacterial and anticancer properties of DAS-AgNPs and garlic extract. The minimum inhibitory concentration required to inhibit Gram negative (Escherichia coli, V. cholera) and Gram positive (S. aureus, B. cereus) were found lesser in DAS-AgNPs followed by garlic extract. Cytotoxicity effect confirmed by DAS-AgNPs and A. sativum towards HeLa cell line by MTT assay. The IC50 values obtained for MTT assay were at 24.09 μg/ml in DAS-AgNPs and 129.22 μg/ml in garlic extract. Anticancer efficacy was found more potent in DAS-AgNPs as compared with aqueous garlic extract.