Abstract
Market analysis has shown that one of the challenges with using enzymes for electrosynthesis is the need for cofactor regeneration of non-redox cofactors (i.e. ATP, GTP, coenzyme A, etc.). Moving from enzymatic bioelectrocatalysis to microbial bioelectrocatalysis is one way to address this problem, but many microorganizations are not designed for the synthesis chemistry your desire and extracellular electron transport is slow. This paper will discuss the use of bioengineering techniques to overexpress synthetic oxidoreductase enzymes in microorganisms simulataneously with outer membrane cytochromes to improve extracellular electron transport. The example system shown will be for nitrogen reduction to ammonia with cyanobacteria.
Published Version
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