Bio-Efficacy of Santan (Ixora coccinea) Leaf Extract against Trichophyton mentagrophytes: An Antifungal Susceptibility Testing

Abstract

This study was conducted to determine the Bio-Efficacy of Santan (Ixora coccinea) Leaf Extract against Trichophyton mentagrophytes: An Antifungal Susceptibility Testing. The study used the experimental research design and subjected the PDA cultures of Trichophyton mentagrophytes into sterile distilled water as a negative control, Ketoconazole as the positive control, and three (3) concentrations of Santan leaf extract in three (3) trials. The study used the two-way analysis of variance as statistical analysis, followed by Bonferroni as a post hoc test. The PDA plate treated with Ketoconazole has 44 millimeters of a zone of inhibition, making it the largest inhibition among all the treatments. There was no zone of inhibition observed on the PDA plate treated with sterile distilled water. The zones of inhibition observed on the PDA plate treated with 100% concentrations of santan leaf extract in three (3) trials are 28mm, 30mm, and 31mm, which are larger than the other concentrations. The PDA plate treated with 75% concentrations of the santan leaf extract has zones of inhibition of 25mm, 27mm, and 28mm. And lastly, the zones of inhibition in the PDA plate subjected to 50% of the santan leaf extract are 21mm, 19mm, and 18mm. Most of the findings exhibited a P-value of < 0.0001, which indicates that there is a significant difference in the antifungal susceptibility of Trichophyton mentagrophytes treated with different concentrations of santan (Ixora coccinea) leaf extract compared with Ketoconazole and distilled water. Although, the santan leaf extract is not as effective as the antifungal agent for Trichophyton mentagrophytes used for positive control, which is Ketoconazole.