Abstract
Within the bioremediation framework, this study was aimed at finding out the optimal amount of sludge waste consumption and microalgal growth conditions of Neochloris oleoabundans in order to reach a significant fat production and chemical transformation of microalgal lipids into biodiesel. The effects of the lipid extraction methods – using ionic liquids and chloroform – on the total amount of fat, the individual fatty acid concentrations, and the biodiesel properties were assessed and compared to the EN 14214 and ASTM D6751−02 biodiesel standards. The microalgae strains grown in the optimized sludge waste medium with a dilution of 0.65 g/L wet sludge, and extracted by ionic liquid:chloroform mixed solvent, resulted being the most significant strains to obtain the highest quality of biodiesel with a density of 0.887 g cm −3 , kinematic viscosity of 4.7 mm 2 s −1 , higher heating value of 40.21 MJ kg −1 , iodine value of 107.3 g I 2 100 g −1 fat, and cetane number of 50. • Sludge waste feedstock was optimized for N. oleoabundans growth. • N. oleoabundans grown in sludge feedstock is suitable for biodiesel production. • Biodiesel properties complied with EN 14214 and ASTM D6751 −02 biodiesel standards.
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