Biodegradation of polypropylene films by Bacillus paralicheniformis and Lysinibacillus fusiformis isolated from municipality solid waste contaminated soil

Abstract

The fossil fuel or petroleum derived plastics are applied in our routine life because of their easy availability. Distribution and contamination of the plastics in the landfills are the major reasons for these biodegradation study. This current study reveals the biodegradation of polypropylene films and the growth of Bacillus paralicheniformis and Lysinibacillus fusiformis isolated from plastic contaminated soil collected from municipality solid waste management site. The degradation rate of PP films was confirmed by the results of biodegradation analysis. The growth of Bacillus paralicheniformis and Lysinibacillus fusiformis had shown OD values at 600nm after the degradation period of 4 weeks increasing from 0.131 to 0.334 and 0.148 to 0.213 respectively. The viable cell count increased from 8×104cells/ml to 12×104cells/ml and 10.1×104 cells/ml to 15.2×104 cells/ml respectively. The physical and chemical changes of PP films were confirmed by FT-IR and XRD analysis. These analysis confirmed that the bacterial strains have the ability to change the chemical and physical nature of PP films and can utilize the PP films as sole carbon source.