Abstract
the degradation rate of chlorothalonil (2,4,5,6-tetrachloroisophthalonitrile) was significantly decreased after repeated application in field experiments. However, the degradation rate completely recovered after further application of chlorothalonil. Chlorothalonil was also degraded in a laboratory study, forming a stoichiometric amount of chloride anion. 4-Hydroxy-2,5,6-trichloroisophthalonitrile (TPN-OH) was also detected as a metabolite in the study. Bacterial which degrade chlorothalonil rapidly were isolated from the soil. The cell suspension of the bacteria transformed chlorothalonil to TPN-OH and chloride anion, but did not utilize chlorothalonil (=cometabolism). It seems that microbial acclimation has occurred during repeated application because the bacteria could not be detected in soil which was not treated with chlorothalonil.
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