Abstract
Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as Sphingobium sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °C and 6.5, respectively. The biodegradation pathway was proposed based on the identification of the metabolites. The addition of cytochrome P450 (CYP) inhibitor 1-aminobenzotriazole significantly decreased the degradation of BPA by Sphingobium sp. YC-JY1. Escherichia coli BL21 (DE3) cells harboring pET28a-bisdAB achieved the ability to degrade BPA. The bisdB gene knockout strain YC-JY1ΔbisdB was unable to degrade BPA indicating that P450bisdB was an essential initiator of BPA metabolism in strain YC-JY1. For BPA polluted soil remediation, strain YC-JY1 considerably stimulated biodegradation of BPA associated with the soil microbial community. These results point out that strain YC-JY1 is a promising microbe for BPA removal and possesses great application potential.
Highlights
Bisphenol A (2, 2-bis (4-hydroxyphenyl) propane, BPA) is extensively used as a plasticizer or flame retardant in the production of synthetic polymers, such as polycarbonate plastics and epoxy resins [1,2,3,4]
The result of Biolog GEN III microplate testing (Table S1) showed that it may be in the same species with Sphingobium paucimobilis B. It suggested that strain YC-JY1 belongs to the genus Sphingobium, it was named as Sphingobium sp
ABT significantly inhibited BPA degradation, and the degradation efficiency decreased when ABT concentration rising, indicating that cytochrome P450 (CYP) was related to BPA degradation in strain YC-JY1. These findings indicated that the CYP is responsible for BPA degradation, which is in accord with some researches showing the involvement of CYP in xenobiotics transformation [37,44]
Summary
Bisphenol A (2, 2-bis (4-hydroxyphenyl) propane, BPA) is extensively used as a plasticizer or flame retardant in the production of synthetic polymers, such as polycarbonate plastics and epoxy resins [1,2,3,4]. A quarter of the BPA was released into the environment during the process of production, transportation, or processing [5,6,7,8]. As a kind of synthetic endocrine-disrupting chemicals (EDCs), BPA would cause reproductive toxicity because of its estrogenic activity [9,10]. Previous studies revealed that BPA could cause carcinogenicity [11], immunotoxicity [12], embryotoxicity [13], and transgenerational influence [14] to various species. The elimination of BPA is essential to the ecosystem and public health
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