Abstract
The development of gene delivery vectors with high efficiency and biocompatibility is one of the critical points of gene therapy. Two biodegradable poly(amino ester)s were synthesized via ring-opening polymerization between low molecular weight (LMW) PEI and diepoxide. The molecular weights of poly(amino ester)s were measured by GPC. Agarose gel retardation assays showed that these materials have good DNA-binding ability and can retard the electrophoretic mobility of plasmid DNA (pDNA) at a weight ratio of 1. The formed polyplexes have proper sizes of around 200 nm and zeta-potential values of about 30–40 mV for cellular uptake. In vitro experiments revealed that polymer P2 gave higher transfection efficiency than PEI 25 KDa and Lipofectamine 2000 with less toxicity, especially in 293 cells. Results demonstrate that such a type of degradable poly(amino ester) may serve as a promising non-viral gene vector.
Highlights
Gene therapy affords a promising way to treat congenital and acquired diseases [1,2]
Results show that the cationic polymer P2 (Mn =n24,550) has a larger molecular weight than than
The degradability of the polymeric material was material was alsoInstudied by Gel permeation chromatography (GPC).hours, In 48the observation hours, the decreased
Summary
Gene therapy affords a promising way to treat congenital and acquired diseases [1,2]. High molecular weight (HMW) PEI, especially with the Mw of 25 KDa, has relatively higher transfection efficiency (TE) both in vitro and in vivo [18] This made it the “golden standard” for the design of six novel polymeric vectors [19,20]. To form a biodegradable cationic polymer is an effective strategy [28,29,30,31,32] These degradable PEIs have shown significantly enhanced transfection activity compared to the parent LMW PEI and retain their biocompatibility due to groups or bonds degradation in cellular environments. Formed hydroxyls and oxygen atoms in in the the backbones backbones could could effectively enhance TE and serum-tolerance similar to PEG structure In in this this report, report, we we synthesized synthesized two kinds of LMW PEI-based biodegradable poly(amino ester).
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