Biodegradable microplastics boost dissimilatory nitrate reduction to ammonium (DNRA) process contributing to ammonium nitrogen retention in farmland soils

Abstract

Microplastics (MPs) pollution in agriculture ecosystems profoundly affects biogeochemical cycles. However, the impacts of MPs, particularly biodegradable MPs, on dissimilatory nitrate reduction to ammonium (DNRA) process remain unclear. Herein, the alterations in denitrification and DNRA pathways and underlying mechanism by different MPs exposures (including conventional nonbiodegradable MPs (polyethylene (PE) and polypropylene (PP)) and biodegradable MPs (polylactic acid (PLA) and polybutylene adipate terephthalate (PBAT)) were explored via soil microcosm experiments accompanied with 15N isotope tracer technology and 16S rRNA high-throughput sequencing. Compared with control, PE and PP increased the rates of denitrification by 117–126% but significantly decreased DNRA rates by 39.3–63.4%, whereas DNRA activities in PLA and PBAT treatments were remarkably boosted by 163–242% and dominated soil nitrate bioreduction (6.67–8.69 μg N g−1 d −1). This differentiation was attributed to the nutrient imbalance induced by MPs and distinctly selective effects of MPs on functional microbes. Microbial interaction mechanism further suggested that limited bioavailable carbon intensified the competition between heterotrophic denitrifiers (e.g., Bacillus, Sphingomonas and Microvirga) and DNRA bacteria (e.g., Luteitalea and Streptomyces) for electron donor in nonbiodegradable MPs treatments, reducing the abundances of DNRA bacteria. However, the high bioavailable C/NOx– ratio resulting from biodegradable MPs degradation released such competition and more greatly promoted the activities of DNRA bacteria than denitrifiers, facilitating ammonium retention in soils. Collectively, this study highlights the promotion of biodegradable MPs on soil ammonium recycle via DNRA process in farmland ecosystems and can shed some light on current plastic mulch-film management toward a sustainable and cleaner agricultural industry.