Abstract
Production of dodecanedioic acid (DDDA), a platform chemical used as raw material for various commodities and polymers, has been studied through a biological process. This process was conducted by using a wild-type of Candida tropicalis which can be obtained easily from natural resources. The aim of this research was to study the characteristics of DDDA production from methyl laurate through batch fermentation process. Growth phase was carried out for 20 h, as the beginning of fermentation, then continued to conversion phase for 5 until 6 days. Utilization of methyl laurate and production of DDDA were analysed using gas chromatography, which proved the ability of C. tropicalis in assimilating methyl laurate to convert it become DDDA. The highest value of cells yield (Yx/s) and DDDA yield (Yp/s) successfully obtained were 0.86 g cells/g methyl laurate and 0.20 g DDDA/g methyl laurate, respectively. This study also showed the possibility of fermentation products accumulation as intermediate, or accumulation of DDDA inside the cells. Thus, this study can be applied as an alternative in addition to the use of mutant microorganism in producing DDDA.
Highlights
The long-chain dicarboxylic acids are well known platform chemicals for production of various commodities, such as polymers, perfumes, hot-melting adhesives, and high quality lubricants [1,2]
This study proved that the use of fatty acid methyl ester (FAME) did not inhibit cell growth
This study has shown that a wild-type of Candida tropicalis is able to produce dodecanedioic acid from methyl laurate
Summary
The long-chain dicarboxylic acids are well known platform chemicals for production of various commodities, such as polymers, perfumes, hot-melting adhesives, and high quality lubricants [1,2]. Crude oil-based DDDAs are still used widely as raw material for polymer production, for example nylon which is produced from crude oil through several steps of chemical reaction [4]. These include limitations in complicated process, generation of unwanted and hazardous byproducts, dependence on non-renewable petrochemical feedstock [1]. Biotechnology based on renewable resources becomes an interesting process, for example by using fatty acids as raw material
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