Abstract
Purple Non-Sulfur Bacteria (PNSB), also known as phototrophic bacteria are widely distributed in both freshwater and marine environment and capable to grow in wide range of substrates. In this study, Bacterium Rhodobacter sphaeroides strain UMS2, a freshwater isolate was used in this study in utilization of fish hatchery waste. This study was conducted to determine the nutritional values of bioprocess product that was grown in fish hatchery waste. Finally, the waste bio-converted product was used as feed supplement to monitor the growth performance of live feed Tubifex spp. Inoculum of Rhodobacter sphaeroides strain UMS2 was developed in 112 synthetic media and 48-h culture of 30% (v/v) inoculum was used in fish hatchery waste during the bioprocess. The nutritional values of bio-converted product, except total ash (%), were not significantly improved with 30% (v/v) inoculum of Rhodobacter sphaeroides, strain UMS2. Feeding trial in bloodworm (Tubifex spp.) with bioconversion product conducted for 15 days to monitor growth (w/v) of live feed. Initial growth 1.42 ± 0.001 g/L of Tubifex spp. was stocked in 15 × 75 × 15 cm plastic tray connected with recirculated system. Tubifex spp. was observed to be comparatively higher (1.55 ± 0.12 g/L) while fed in the product that contained bacterium than the growth (1.44 ± 0.15 g/L) of Tubifex spp. fed in the bioconversion product of without bacterium. The inoculums size (30%) of bacterium not enough to support the growth of Rhodobacter sphaeroides, strain UMS2 in the bioconversion process to improve the nutritional values. However, while used as feed supplement it improved the growth performance of the Tubifex spp. So, bacterium Rhodobacter sphaeroides, strain UMS2 has potentiality to be used as feed supplement in the production of live feed.
Highlights
The waste generated from fish hatchery mainly contained 7% - 32% of the total nitrogen and 30% - 84% of the total phosphorus [1]
Inoculum of Rhodobacter sphaeroides strain UMS2 was developed in 112 synthetic media and 48-h culture of 30% (v/v) inoculum was used in fish hatchery waste during the bioprocess
Ash and crude protein are the major component obtained in Hatchery Waste Powder (HWP) was used in the bioprocess (Table 1)
Summary
The waste generated from fish hatchery mainly contained 7% - 32% of the total nitrogen and 30% - 84% of the total phosphorus [1]. The conventional method for the treatment of wastewater in a recirculation process which includes solids removal, ammonia oxidation, aeration and disinfection [1]. Two types of waste generated from UMS finfish hatchery, soluble and solid waste and they are both organic and inorganic. The wastewater from UMS finfish hatchery is not different from that of a production farm in terms of quality and quantity of waste. The wastewater from UMS finfish hatchery generated from larval rearing and fry production units, and from other culture units [2]. It is necessary to develop new research application of converted this waste to a value-added product rather than to use uneconomic conventional method for treatment
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