Abstract
In these studies concentrated sugar solutions of barley straw and corn stover hydrolysates were fermented using Clostridium beijerinckii P260 with simultaneous product recovery and compared with the performance of a control glucose batch fermentation process. The control glucose batch fermentation resulted in the production of 23.25gL−1 ABE from 55.7gL−1 glucose solution resulting in an ABE productivity and yield of 0.33gL−1h−1 and 0.42, respectively. The control reactor (I) was started with 62.5gL−1 initial glucose and the culture left 6.8gL−1 unused sugar due to butanol toxicity resulting in incomplete sugar utilization. Barley straw (BS) hydrolysate sugars (90.3gL−1) resulted in the production of 47.20gL−1 ABE with a productivity of 0.60gL−1h−1 and a yield of 0.42. Fermentation of corn stover (CS) hydrolysate sugars (93.1gL−1) produced 50.14gL−1 ABE with a yield of 0.43 and a productivity of 0.70gL−1h−1. These productivities are 182–212% higher than the control run. The culture was able to use 99.4–100% sugars (CS & BS respectively) present in these hydrolysates and improve productivities which were possible due to simultaneous product removal. Use of >100gL−1 hydrolysate sugars was not considered as it would have been toxic to the culture in the integrated (simultaneous fermentation and recovery) process.
Published Version
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