Abstract
The biocontrol of plant diseases by microorganisms is a promising alternative to the chemical pesticides. Serratia marcescens strain B2 effectively controls fungal diseases of cyclamen and rice. Biocontrol by S. marcescens strain B2 is mediated by the combined effects of plural chitinases, antibiotic prodigiosin, induced systemic resistance. Activity of S. marcescens is often negatively affected by abiotic and biotic factors and antibiotic biosynthesis of this bacterium is reduced under the influence of rice-associated bacteria. A genetically modified rice-indigenous bacterium was developed by introducing genes encoding for antifungal factors. Disease inhibitory genes were isolated from S. marcescens and put under the control of several types of promoters, which were isolated from the recipient. These genetically modified microorganisms effectively suppressed rice blast disease caused by Pyricularia oryzae and are not affected by abiotic or biotic factors. Introduction of disease inhibitory genes controlled by promoters and derived from the recipient is a useful technology for the development of biocontrol agents.
Published Version
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