Biocompatible synthesis of silver nanoparticles (AgNPs) using marine Vibrio sp. and study their pharmacological applications

Abstract

This report presents a rapid and a green biogenic approach for the biosynthesis of silver nanoparticles using marine bacteria such as Vibrio sp. The bioactive compounds of Vibrio sp. induced the reduction of Ag+ ions from AgNO 3 , which resulted in the formation of bacterial mediated silver nanoparticles (AgNPs). The reduction of Ag+ ions to elemental silver was characterized by UV-vis spectroscopy, Fourier transform infra-red spectroscopy (FTIR), Scanning electron microscopy (SEM) and Dynamic light scattering (DLS). The nanoparticles show maximum absorbance at 421 nm on ultraviolet-visible spectra. The presence of biomolecules were identified by Fourier transforminfrared spectroscopy. The bioactive compounds and nanoparticles were found to direct different shape and sized and their size/ shape were varied. The silver nanoparticle synthesized were spherical, in the range of 40-80 nm in size. In antimicrobial screening, all the pathogens were fairly affected and nil effect was not observed in the test samples, In bacteria, the test sample was most effective against Enterococcus faecalis B2 and Salmonella typhimurium B5. In fungi, which was effective against Candida albicans F1, Microsporum canis F3 and Trichophyton rubrum F4.