BIOCOMPATIBLE COMPOSITES WITH LYSOZYME BASED ON POLYURETHANE UREA WITH N-VINYL PYRROLIDONE COPOLYMER FRAGMENTS, VINYL ACETATE AND VINYL ALCOHOL

Abstract

A series of PUS containing a N-vinylpyrrolidone-vinyl alcohol copolymer (VP-VA) copolymer with different percentages of 4,4’-diaminodiphenylmethane (DADF) to VP-VA (30:70; 50:50; 70:30) was synthesized. On the basis of synthesized PUS, a number of composite materials containing lysozyme (the content of the medicinal substance is 1 wt%) was obtained.According to IR spectroscopic studies, immobilization of lysozyme occurs due to intermolecular hydrogen bonds (physical immobilization).Variation of the content of copolymer VP-VA in the structure of the received РUS causes changes of physical-mechanical properties. It was established that with decrease in the amount of VP-VS copolymer in the structure of PUS there is an increase in strength and relative elongation at breakIt was established that samples of PUS with fragments of VP-VS in their structure, filled with lysozyme, synthesized by the percentage ratio of DADF to copolymer VP-VS 70:30 have the best physical and mechanical properties in a series (σ = 11,5 MPa, ε = 76%). The introduction of the resulting polymer materials lysozyme leads to increased strength at break from 43% to 97%.The thermophysical properties of PUS depend on the presence of lysozyme in their composition.When introducing into the structure PUS of the enzyme , an increase in the glass transition temperature and a decrease in the heat capacity jump due to the decrease in the segmental mobility of the macromolecules. It is established that the studied systems are single-phase.The method of culture of tissues was founding that all composite materials are biocompatible and non-toxic. For experimental samples with lysozyme, more active growth of fibroblast cells was observed.