Biocompatibility of a quaternary ammonium derivative of pectin with rabbit's bone mesenchymal stem cells

Abstract

Objective To study the biocompatibility of a quaternary ammonium derivative of pectin with rabbit' s bone mesenchymal stem cells (BMSCs).Methods The rabbit' s BMSCs were co-cultured with a quaternary ammonium derivative of pectin in vitro,and the cell attachment,cell proliferation rate and ALP activity were detected.Results The BMSCs could be attached to the quaternary ammonium derivative of pectin.In cell proliferation rate,there was no significant difference between the experimental group and control group at 3rd day (0.281 ±0.003 vs.0.204 ±-0.013) and 6th day (0.513 ±0.016 vs.0.583 ±0.031,P ＞ 0.05),but at 9th day,there was significant difference between two groups (1.108 ± 0.124 vs.0.645 ±0.049,P ＜0.05).ALP activity was significantly higher in experimental group than in control group at each time point (P ＜ 0.05).Conclusion The quaternary ammonium derivative of pectin has good biocompatibility with rabbit' s BMSCs,and could be used to repair bone defects as a biomaterial in tissue engineering. Key words: Tissue engineering; Bone mesenchymal stem cells; Quaternary ammonium derivative of pectin; Biocompatibility