Effect of growth factors-coated electrospun nanofibrous films on the growth of bone marrow mesenchymal stem cells

Abstract

Objective To explore the impact of growth factors-coated electrospun [poly(L-lactideco-e-caprolactone) (PLCL)]/fibrinogen nanofibrous films on the growth of rabbit bone marrow mesenchymal stem cells (BMSCs).Methods Electrospun nanofibrous films were fabricated from PLCL solution and fibrinogen solution.The nanofibrous films which were immersed into fibronectin solution and growth factors solution [vascular endothelial growth factor (VEGF),basic fibroblast growth factor (bFGF)] sequentially served as experimental group A films,those which were immersed into growth factors solution only as experimental group B films,and those without given any treatment as control group.The in vitro release results of growth factors in groups A and B were detected by using enzyme-linked immunosorbent assay (ELISA).BMSCs were obtained from rabbits by density gradient centrifugation and inoculated on the films.The adhesion and proliferation results of BMSCs on different films were detected by methyl thiazol tetrazolium (MTT) assay.Results In both experimental groups A and B,the growth factors were released in vitro for over 10 days,the released amount of the growth factor in the two groups was gradually reduced with time,and the reduction rate in group B was higher than in group A.The absorbance values in group B (0.37 ±0.06) and control group (0.35 ±0.09) were significantly lower than in group A (0.47 ±0.04) during the first 12 h (P ＜ 0.01).The cell proliferation rate was higher in group A than in group B and control group (P ＜ 0.05).Conclusion Electrospun PLCL/fibrinogen nanofibrous films,which surface is modified by fibronectin and growth factors (VEGF,bFGF) sequentially,have good performance in growth factors sustained release and cell compatibility. Key words: Bone marrow mesenchymal stem cells ; Electrospinning; Nanofibrous; Surface modification ; Tissue engineering