Biocompatibility assessment of silver chloride nanoparticles derived from Padina gymnospora and its therapeutic potential

Abstract

The objective of the present work was to improve the biological activity of Padina gymnospora. In the current study, silver chloride nanoparticles have been synthesized using the aqueous extract of Padina gymnospora and further characterized by ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, x-ray powder diffraction, scanning electron microscopy, energy dispersive spectroscopy, transmission electron microscopy and atomic force microscope. Further, the hemolytic activity and eco-toxicity of silver chloride nanoparticles analyzed. The synthesized silver chloride nanoparticles were found to be mono-dispersed and spherical with an average size of 11.5–32.86 nm. The particles showed an anticancer effect in a dose-dependent manner against breast cancer cell line (MCF-7 cell lines) (IC50 = 31.37 μg ml−1). In addition, it showed the larvicidal activity against Aedes aegypti at a lower dose (3.92 μg ml−1) than that of the aqueous extract (13.01 μg ml−1). Nanoparticles also exhibited greater antimicrobial activity for both bacterial and fungal pathogens. The synthesized silver chloride nanoparticles showed a maximum zone of inhibition, i.e., 31 mm for Candida albicans followed and 27 mm for vancomycin resistance Enterococcus faecalis.The results suggest the possible use of synthesized silver nanoparticles with P. gymnospora as therapeutic agent for breast cancer, dengue vector control and as antimicrobial agent.