Abstract
Mitochondria from neonatal and adult rat brains and also from adult rat liver were compared for their ability to oxidize isocitrate in the presence and absence of added pyridine nucleotides and nicotinamide. Nicotinamide enhanced the oxidation of isocitrate by adult rat liver and brain mitochondria in the presence of added NAD and depressed it in the presence of added NADP. Phosphorylation associated with the oxidation of isocitrate was also affected by nicotinamide, depending on the added coenzyme and the tissue under consideration. Nicotinamide enhanced the activity of NAD-isocitrate dehydrogenase (EC 1.1.1.41) and inhibited the activity of NADP-isocitrate dehydrogenase (EC 1.1.1.42) and NADH2-cytochrome c reductase (EC 1.6.2.1) of brain mitochondria. It had no effect on NADPH2-cytochrome c reductase (EC 1.6.2.3).
Published Version
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