Abstract
The incorporation of adenine- 8-C 14 into the nucleic acids of virus-infected cells was studied in the system of vaccinia virus (strain V-MH) growing in monolayers of HeLa cells. All cells were infected and yielded an average of 100 pock-forming units of virus per cell in 30 hours. The tracer was incorporated into both nuclear and cytoplasmic RNA. During the early stages of infection the rate of incorporation of adenine into the microsomal fraction of infected cells was significantly greater than that into the corresponding fraction from normal cells. The maximum was reached at about the time when the first new virus particles appeared; as cellular damage became marked, the rate of incorporation decreased. The effect of certain variables such as the length of the infection period, the duration of exposure to adenine, the multiplicity of infection, and the composition of the medium were studied. No significant increase in either RNA or DNA could be demonstrated. The pattern of the incorporation in relation to the time scale of virus multiplication and the distribution of the label among cell fractions is discussed.
Published Version
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