Biochemical responses of the desiccation-tolerant resurrection fern Pleopeltis polypodioides to dehydration and rehydration

Abstract

The epiphytic fern Pleopeltis polypodioides can tolerate repeated drying and rehydration events without conspicuous damage. To understand the biochemical principles of drought-tolerance, we analyzed the effect of dehydration and rehydration at 25 °C on hydroperoxide and lipid hydroperoxide, the activities of antioxidative (catalase and glutathione-oxidizing) enzymes and evaluated changes in fatty acid composition and saturation levels. Dehydration increased peroxide concentration and the activity of glutathione oxidases, but reduced catalase activity. During dehydration, the biosynthesis of palmitic (C16:0), linoleic (C18:2), linolenic (C18:3) and stearic acid (C18:0) increased 18, 12, 20, and 8-fold, respectively. In contrast, rehydration lowered levels of peroxides, the activity of glutathione-oxidizing enzymes, and fatty acids but increased catalase activity. The coordinated changes during de- and rehydration suggest that lipids and oxidative and antioxidative enzymes are components of the drought-resistance system.