Abstract
Biochemical properties of cytochrome b5-dependent microsomal fatty acid elongation and identification of products.
Highlights
In the presence of malonyl-CoA and malonyl-CoA in- the general outline of microsomal elongation has corporation measured under various conditions was demonstrated, providing further evidence in support of our hypothesis
We found that the rate of bs reoxidation increased in the presence of malonyl-CoA plus ATP and that malonyl-CoA incorporation was inhibited by ananti-cytochrome b5 IgG
When using [1,3-’4C]malonylCoA for incorporation experiments, it was noticed that a substantial amount of radioactivity was extracted, if NADH was omitted from the reaction
Summary
M sucrose and by Ca2+precipitation (15).The final microsomal pellet was resuspended in 50 mM Tris-HC1, pH 7.4, containing 0.15 M KCl. Measurement of Cytochrome bg Reoxidation Rates-The reaction mixture used for measuring cytochrome b5 reoxidation rates in a spectrophotometric cuvette consisted of the following components (fial concentration): 100 m Tris-HC1, pH 7.4, 2 p~ rotenone, 500
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