Abstract
Summary 18 methanol negative mutants of Acetobacter methanolicus MB 58 induced by MNNG or UV light were tested for enzymes of the linear and the cyclic dissimilatory sequence of formaldehyde as well as of phosphofructokinase. Most of the mutants are pleiotrophic ones. The highest proportion of defects was found in C1 regulated methanol dehydrogenase and in constitutive formaldehyde dehydrogenase activity. The cytochrome c deficiency was always combined with that of the methanol dehydrogenase. Formate dehydrogenase was only absent in one pleiotrophic mutant. In none of the heterotrophically grown mutants any enzyme of the dissimilatory RuMP cycle as well as phosphofructokinase were defective. Hexulose-6-phosphate isomerase could not be further induced by methanol in 3 methanol oxidation positive mutants indicating the existance of isoenzymes. No enzyme lesion was detected in 2 mutants which should be defective in other assimilatory enzymes.
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