Abstract
Thirty cotton genotypes (5 Gossypium arboreum and 25 Gossypium hirsutum) were analyzed for biochemical characters related to drought tolerance. Genotypes, JDRL-1, JDRL-2, JDRL-3, JDRL-4, JDRL-5 (G. arboreum) and JDRL-6 (G. hirsutum) were found to be highly drought tolerant based on relative water content (RWC) under stress condition. However, JDSL-24 (G. hirsutum) was recorded highly drought susceptible. Among biochemical parameters, soluble proteins correlated positively against drought tolerance (RWC) followed by proline and glycine betaine content. Leaf soluble proteins (15.35 mg×g−1fr.wt.), proline content (0.20 mg×g−1fr.wt.) and glycine betaine content (9.64 mg×g−1fr.wt.) was recorded highest in drought tolerant genotype JDRL-1 while free amino acids (0.470 mg×g−1fr.wt.) were elevated in JDRL-3. Total 20 RAPD primers were used to generate 198 bands in which 188 bands were polymorphic with an average of 9.4 bands per primer and total six RAPD primers [CPA-11(2738, 672, 376), OPA-2(502), OPE-08(4375, 3724, 488), OPM-06(407), OPO-09(2726, 2102) and OPZ-15(2211, 1124, 276)] amplified shared unique fragments between all five highly drought tolerantG. arboreum and two tolerant G. hirsutum genotypes JDRL-6 and JDRL-15. Dendrogram constructed from the RAPD data showed that JDRL-4 genotype of cotton was the most diversified genotype observed having separate position in cluster-II. The leaf osmolytes glycine betaine, soluble proteins, free amino acid and free proline were the effective biochemical markers to screen the cotton genotypes suitable for drought condition.
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