Abstract
In this study, the third instar larvae of Culex pipiens Linnaeus, 1758 were treated with a lethal concentration of the essential oil (EO) of Citrus sinensis (L.) Osbeck; its nanoemulsion and the EO dissolved in the absolute ethanol were applied. The tested EO induced biochemical and histological changes in the treated larvae. The biochemical changes were presented by an increase in the levels of the acid phosphatase and alkaline phosphatase and non-significant change in the activity of acetylcholinesterase and glutathione S-transferase. Histological studies revealed obvious alterations in the cuticle, muscles, and midgut tissues including changes on the ultrastructural level.
Published Version
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