Biochemical analysis of some fruit peels and comparison of Lactic acid production by authochthonous Lactic acid bacteria using fruit peels

M. O. Arekemase,M. I. Ahmed,D. O. Adetitun

doi:10.4038/sljb.v5i1.53

Abstract

Waste to wealth is a common trend in the world today. This work reports the utilization of waste fruit peels for production of lactic acid using authochthonouslactic acid bacteria. Peels of mango, orange and banana were prepared in powdered form, pretreated with HCl and added as carbon source for lactic acid production. The proximate analyses of the substrates were done. The organisms used for fermentation were isolated from spoilt banana and identified using biochemical and molecular tools. These organisms were grown using different growth factors over a period of eight days with initial pH of 6.0. Growth factors were then varied in order to optimize the yield of lactic acid. Percentage production of lactic acid was determined every 24 hours. The optimized conditions were then combined for further comparison. Isolated Lactobacillus plantarumand Lactobacillus caseiwere used for the production of lactic acid. The carbohydrate content of mango, banana and orange peels (62.48%, 55.34% and 57.06% respectively) showed that it was sufficient for use as a carbon source. The highest yield of lactic acid (27.10%) was at pH 6.0 with 2g of substrate and 1 ml inoculum at day six by Lactobacillus caseiwhile the highest yield by Lactobacillus plantarum (23.85%) was at pH 6.0, with 4g of substrate and 2 ml inoculum at day six. The highest production of lactic acid was derived when mango peels were used. The results of this study showed that lactic acid can be produced using mango, orange and banana peels.

Highlights

Fruit based industry produces a large volume of solid and liquid waste
Isolation and Identification of test organisms The use of autochthonous lactic acid bacteria (LAB) for the production of cheese and organic acid was reported by Renes et al, (2019)
The pH range used in this work was 5 to 6.5 with highest yield obtained at pH 5 and 6 respectively for L. plantarum and L. casei

Summary

Introduction

Fruit based industry produces a large volume of solid and liquid waste These pose increasing disposal and pollution problems (high BOD or COD) and represents loss of valuable biomass and nutrients (Mridul&Preethi, 2014).Disposal of wastes from fruit-canning industries has been a problem due to high transportation costs and limited availability of landfills, as these byproducts carry no commercial value, they are often discharged in places they ought not to be disposed (Pradeep et al, 2014). This occurs where there is a lack of legislation and their enforcement on waste disposal (Omojasolaet al., 2009). The main use of such domestic food waste is the production of valuable compounds by the controlled break down of the waste by microorganisms (Rounsefellet al., 2013)

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