Biochemical Alterations in Reproductive and Hepatorenal Functions in Male Rabbits Supplemented with Margarine, Natural Ghee and Olive Oil

Abstract

The current study investigated the impact of synthetic margarine, natural ghee and olive oil ‎on the biochemical parameters of male rabbits, focusing on reproductive hormones as free ‎testosterone, bioavailable testosterone, sex hormone binding globulin (SHBG) also body ‎weight gain, antioxidant markers, hepatic and kidney functions. Forty male New Zealand ‎rabbits were randomly distributed to four groups, each containing 10 rabbits. The control ‎group was supplemented with the basal diet, while the margarine group was received 10% ‎margarine, the ghee group was fed on 10% natural ghee of diet and the olive oil group ‎received diet with 10% olive oil. Results indicated that the administration of synthetic ‎margarine throughout the experiment significantly increased body weight gain, liver enzyme ‎‎(AST), and renal profile (urea) in comparison to other groups. Moreover, there was a ‎decrease in reproductive hormones as free and bioavailable testosterone, and antioxidant ‎enzyme activity (GPx) in Margarine group; meanwhile, these modified parameters were ‎enhanced in ghee group and olive oil group. This study concluded that olive oil and natural ‎ghee had a more beneficial health impact on fertility performance compared to synthetic ‎margarine.‎