Biochar Influences Polyethylene Microplastic-Contaminated Soil Properties and Enzyme Activities

Abstract

Microplastics (MPs) have emerged as a significant pollutant, threatening agricultural ecosystem sustainability and global food security. However, relatively few studies have investigated biochar remedial effects on plant growth and biochemical properties in soils contaminated with MPs. In polyethylene microplastic (PE-MP)-contaminated soil, we investigated corn stover biochar effects on soybean growth, soil nutrient content, enzyme activity, and microbial biomass and assessed its impact on soil microbial resource limitations. The addition of MPs inhibited soybean growth in various forms across four stages. Conversely, the addition of biochar to MPs improved soybean growth to some extent, where above-ground biomass increased by 5.82% after adding biochar to soils containing microplastics. In soil treated with MPs and biochar, nitrate nitrogen (N), available phosphorus (P), and available potassium (K) increased by 20.1, 27.4, and 57.2%, respectively, while available nitrogen significantly decreased to 128.3 mg kg−1 compared to the MP-only treatment. PE-MPs alone significantly reduced soil carbon (C), N, and P enzyme activities, as well as microbial biomass, with β-glucosidase, leucine aminopeptidase, and acid phosphatase activities decreasing by 29.9, 27.8, and 25.5%, respectively. Interestingly, biochar addition to MPs significantly alleviated these detrimental effects. Microbial biomass C, N, and P increased by up to 56.0, 22.5, and 96.6%, respectively, following biochar addition to soils containing MPs. Analysis of vector lengths, vector angles, and scatter plots indicated that the presence of MPs reduced soil N and P availability. Overall, while MPs inhibited soybean development, biochar addition alleviated this effect to some extent. Furthermore, partial least-squares path modeling revealed that MPs negatively affected soil chemical properties, microbial biomass, and enzyme activities, whereas biochar positively influenced soil enzyme activities.