Biochar amendment modulates xylem ionic constituents and ABA signaling: its implications in enhancing water-use efficiency of maize (Zea mays L.) under reduced irrigation regimes

Abstract

While biochar amendment is known to enhance plant productivity and water-use efficiency (WUE), particularly under water-limited conditions, the specific mechanisms driving these benefits remain largely elusive. Thus, the present study was conducted to elucidate the synergistic effects of biochar and reduced irrigation on maize (Zea mays L.) plants, focusing on xylem composition, root-to-shoot signaling, stomatal behavior, and WUE. Maize plants were cultivated in split-root pots filled with clay loam soil, amended by either wheat-straw (WSB) or softwood (SWB) biochar at 2% (w/w). Plants received full (FI), deficit (DI), or alternate partial root-zone drying (PRD) irrigation from the fourth leaf to the grain-filling stage. Our results revealed that WSB amendment significantly enhanced plant water status, biomass accumulation, and WUE under reduced irrigation, particularly when combined with PRD. Although reduced irrigation inhibited photosynthesis, it enhanced WUE by modulating stomatal morphology and conductance. Biochar amendment combined with reduced irrigation significantly increased xylem K+, Ca2+, Mg2+, NO3-, Cl-, PO43-, and SO42- while decreased Na+, which in turn lowered xylem pH. Moreover, biochar amendment and especially WSB amendment further resulted in increased abscisic acid (ABA) contents in both leaf and xylem sap under reduced irrigation conditions due to changes in xylem ionic constituents and pH. The synergistic interactions between xylem components and ABA led to refined adjustments in stomatal size and density, thereby affecting stomatal conductance and ultimately improving WUE of maize plants at different scales. The combined application of WSB and PRD can therefore emerge as a promising approach for improving the overall plant performance of maize plants with increased stomatal adaptations and WUE especially under water-limited conditions.