Abstract
Mycotoxins pose significant health risks due to their prevalence in food products and severe health implications, including carcinogenicity. This study investigates the bioavailability of mycotoxins aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEN) individually and combined, in the presence of identified polyphenols from tiger nut beverage (TNB) and tiger nut by-product (TNBP) using the in vitro model Caco-2 cells, which simulates the human intestinal barrier. The objective is to understand how bioactive compounds from TNBP can mitigate the effects of AFB1, OTA and ZEN (and their combination) by bioavailability interference, contributing to safer food products and innovative food safety strategies. In vitro gastrointestinal digestion was simulated using the INFOGEST protocol, followed by a bioavailability assessment through transepithelial transport assays in differentiated Caco-2 cells. OTA bioavailability significantly increased in the presence of TNB and TNBP, suggesting interactions that enhance its intestinal absorption. AFB1 maintained high bioavailability across all conditions (up to 83%), while ZEN showed a general decrease (up to 24%), thus indicating a potential protective effect of TNB and TNBP against ZEN toxicity. Regarding the effect of mycotoxins on the bioavailability of polyphenols from TNB and TNBP, a general enhancement was observed for TNB consistently showing higher bioavailability than for TNBP. Notably, OTA and ZEN significantly increased polyphenols bioavailability, reaching up to 79.2% in TNB. Individual polyphenol generally showed a notable reduction in trans-ferulic acid and an increase in trans-cinnamic acid in the presence of mycotoxins. For TNBP, individual mycotoxins generally enhanced polyphenol bioavailability, with AFB1 showing the most significant increase. In conclusion, tiger nut products show promise as sources of bioactive compounds for mitigating mycotoxin toxicity in food products. However, further studies are necessary to clarify these interactions and optimize the conditions of use for their safe and effective application in the food industry.
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