Abstract
Laboratory bioassays demonstrated that formulation of artificial insect diet influenced the expression of antibiosis to fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), in grain sorghum, Sorghum bicolor (L.) Moench. Larvae of FAW were reared on a diet formulated with pinto bean and a diet formulated with no pinto bean, both supplemented with dried milk stage florets of resistant and susceptible sorghum genotypes. The sorghum lines showed a significantly higher antibiosis to FAW when mixed in the diet formulated with no pinto bean than when mixed in the diet containing the bean. Larvae that were fed the no-bean diet supplemented with resistant sorghum genotypes weighed one-half to one-third less, required longer to pupate, and resulted in lighter pupae than larvae reared on mixtures containing the bean. The results obtained when the larvae were reared on the bean diet were significantly correlated (P = 0.0001, r > 0.80, n = 18) with those recorded when the no-bean diet was used for the FAW variables measured. Because a higher expression of antibiosis is obtained with the no-bean diet, this formulation should be recommended for bioassays designed to study lines with low levels of resistance to insects or to screen plant introductions in which the level of resistance is not known.
Published Version
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