Abstract
The anabolic androgenic steroids (AAS) are the most frequently consumed performance enhancing drugs (PED) in sports. In the anti-doping field, the detection of AAS is carried out by the analysis of the athlete’s urine using methodologies based on liquid/gas chromatography-mass spectrometry. Unfortunately, the detection of unknown compounds is not possible. BDS’s AR CALUX® bio detection technology was studied as an indirect method to detect the administration of a single dose of testosterone (T). Twelve T and placebo single dose administered men volunteers underwent a triple-blind crossover clinical trial. The UGT2B17 deletion was present among the volunteers and evenly distributed in heterozygous (ins/del), wild-type homozygous (ins/ins), and mutated homozygous (del/del) groups. A significant statistical difference in terms of bioluminescence was observed after the testosterone (T) administration for the three types of polymorphic groups. The ratio of means between the pre- and post-T administration periods, depending on the type of polymorphism, was in group ins/ins 3.31 (CI. 95%: 2.07–5.29), group ins/del 4.15 (CI 95%: 3.05–5.67), and group del/del 2.89 (CI 95%: 2.42–3.46). The results of the study are very promising, as they may offer us the possibility of designing a detection approach that, based on intra-individual monitoring of androgenic values, in the UGT2B17 deletion type.
Highlights
IntroductionSport plays an important role in society due to the values of effort, dedication, and excellence that it transmits
12 individuals participated in the study, but due to the inconvariables are expressed as their mean and standard deviation (SD) and variables not sistency and dispersion of the results compared to the rest, it was found that most of the showing a normal distribution as medians and interquartile ranges (IQR = P25–P75)
In the case of the samples that were not submitted to hydrolysis, due to the fact that the androgenic response is always much weaker, six samples from two individuals had to be discarded due to the practical absence of signal; for the non-hydrolyzed fraction, the total number of samples available was 252
Summary
Sport plays an important role in society due to the values of effort, dedication, and excellence that it transmits. It is very often tainted by the fraudulent use of doping substances to enhance athletic performance and to perform under the pressures of success. This disrupts fair play and constitutes a serious health risk for athletes [1,2]. Steroids are the substances most widely detected by WADA accredited laboratories [3]. Approaches for the detection of steroid abuse in the Anti-Doping field are standardized by the World
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