Bioactivities of Phenolic Compounds from Kiwifruit and Persimmon

Young-Mo Kim,Kyung-Sik Ham,Seong-Gook Kang,Yong-Seo Park,Martyna Lubinska-Szczygeł,Faridah Abas,Yang-Kyun Park,Shela Gorinstein,Aviva Ezra

doi:10.3390/molecules26154405

Abstract

Fruit used in the common human diet in general, and kiwifruit and persimmon particularly, displays health properties in the prevention of heart disease. This study describes a combination of bioactivity, multivariate data analyses and fluorescence measurements for the differentiating of kiwifruit and persimmon, their quenching and antioxidant properties. The metabolic differences are shown, as well in the results of bioactivities and antioxidant capacities determined by ABTS, FRAP, CUPRAC and DPPH assays. To complement the bioactivity of these fruits, the quenching properties between extracted polyphenols and human serum proteins were determined by 3D-fluorescence spectroscopy studies. These properties of the extracted polyphenols in interaction with the main serum proteins in the human metabolism (human serum albumin (HSA), α-β-globulin (α-β G) and fibrinogen (Fgn)), showed that kiwifruit was more reactive than persimmon. There was a direct correlation between the quenching properties of the polyphenols of the investigated fruits with serum human proteins, their relative quantification and bioactivity. The results of metabolites and fluorescence quenching show that these fruits possess multiple properties that have a great potential to be used in industry with emphasis on the formulation of functional foods and in the pharmaceutical industry. Based on the quenching properties of human serum proteins with polyphenols and recent reports in vivo on human studies, we hypothesize that HSA, α-β G and Fgn will be predictors of coronary artery disease (CAD).

Highlights

Antioxidant activities of bioactive compounds for human nutrition and health are directly connected with different fruits, such as exotic and traditional, which are commonly used in daily consumption [1,2]
Each batch was composed of 25 fruits, about two kg in weight
The samples were washed with tap water and dried

Summary

Introduction

Antioxidant activities of bioactive compounds for human nutrition and health are directly connected with different fruits, such as exotic and traditional, which are commonly used in daily consumption [1,2]. Nuclear magnetic resonance (NMR) has increasingly become an attractive tool in metabolomics analysis and has been combined with multivariate data analysis such as principal component analysis (PCA) and partial least-squares discriminate analysis (PLS-DA) in order to evaluate the properties of the bioactivity of compounds, especially phenolic ones with antioxidant properties. This approach has been used to identify differences among varieties of foods, the quality of cultivar selection, and taste evaluation [9,10,11,12]. While reports on polyphenol bioavailability have increased, there is still limited knowledge about the dynamics of polyphenol metabolism [18,19,20]

Methods

Results

Conclusion