Bioactive phytochemicals in Annona muricata fruit juice ethanol extract

Abstract

Phytochemical contents of Annona muricata fruit juice ethanol extract was determined using gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). Lyophilized fruit juices were extracted in 10 % v/v aqueous solution of ethanol at 60 0C in a water bath. The GC-MS screening of the extract revealed the concentrations (mg/ml) of thirty-three (33) phytochemicals which included glycerine (9.12), levoglucosenone (5.18), 1,3-propanediol-2-(hydroxymethyl)-2-nitrobenzene (11.83) and D- allose (7.28), benzofuran-2,3-diydro-(1.87), 3,7,11,15-tetramethyl-2-hexadecen-1-ol (1.52), Cis-11,14-Eicosadienoic acid, methyl ester (1.53), 9-Octadecenoic acid, methyl ester (1.68), 2-Methoxy-4-vinylphenol (1.69), which are reported to exhibit various biological activities. The HPLC analyses of the extract revealed various polyphenolic compounds (mg/ml) such as naringenin (12.97), proanthocyanin (0.23), tannin (44.17), coumaric acid (4.12), catechin (39.20), resveratrol (42.27) and kaempferol (29.86). which also possess various health benefits like antibacterial, antifungal, anti-inflammatory, anticancer, antimutagenic, antioxidant, antitumor, and others. It can be concluded that the Annona muricata fruit juice ethanol extract contained bioactive phytochemicals that exhibit promising health-promoting activities.