Abstract
In this study, we prepared a biocomposite made of decellularized human amniotic membrane (dHAM) and mesoporous bioactive glasses (MBGs). dHAM was solubilized by using pepsin and acetic acid and then impregnated with copper- and cobalt-doped 13-93B3 MBGs (CuCo-MBGs). The prepared composition was then lyophilized to prepare biocomposite membranes. SEM displayed the homogenous presence of glass particles inside dHAM. The composites showed compatibility with fibroblast cells. dHAM + MBG/CuCo-MBG had higher migratory effects on human umbilical vein endothelial cells (HUVECs) compared to glass-free samples. The angiogenic activity of the samples was further confirmed by the chicken chorioallantoic membrane angiogenesis (CAM) assay. The incorporation of MBG and CuCo-MBG into dHAM resulted in enhancing the antibacterial activity. In conclusion, Cu/Co-doped MBGs could improve the biological functions of dHAM without any adverse significant effects in vitro. In conclusion, this composite could be considered the next generation of wound dressings after performing in vivo experiments.
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