Abstract
Studying on endophytic actinomycetes of Cinnamomum sp. from Indian rainforest, Cherapunji, one of our isolated strains designated as CH1 was found to produce significant antibacterial activity against test pathogens (Aeromonas caviae (ATCC 15468), Vibrio parahemolyticus ATCC 1782, Pseudomonas aeruginosa (ATCC 9027), Proteus vulgaris (ATCC 12453), Shigella flexnerii (ATCC 12022), Escherichia coli (human sample isolate), Bacillus subtilis (ATCC 11774) and Bacillus cereus (ATCC 14579). Scanning electron microscopic studies, morphological characterization and 16s ribosomal RNA gene sequence based classification strongly suggest that the isolate is a new strain of Streptomyces rochei. The rRNA gene sequenced was submitted to NCBI (GenBank accession number KJ486840.1). An antibacterial metabolite was extracted from fermented culture broth and purified by repeated silica gel column chromatography. TLC and bioautogram study of active fraction showed compound of Rf 0.196 was active metabolite where as HPLC study showed two major peak of RT 2.433 and 2.632 min. Parameters influencing optimal antimicrobial production were also determined and the active compound was found nonmutagenic by Ames test. Based on above experiments it is concluded that this entophytic isolate can be further exploited for industrial and biological applications.
Highlights
The emerging field of antimicrobial therapy in recent decades has cultivated much interest in using microbial natural products, such as toxins, proteins, hormones, vitamins and amino acids for diseases control (Fatope et al, 2000)
In recent years more than 90% antibiotics have obtained from actinobacteria and among which about 55% belong to the genus Streptomyces (Hamaki et al, 2005)
Other morphological and physiochemical properties of strain CH1 was summarized in Table 1. 16s rRNA sequences and phylogeny study showed close relationship between CH1 and various type strains of Streptomyces rochei (Fig. 2)
Summary
The emerging field of antimicrobial therapy in recent decades has cultivated much interest in using microbial natural products, such as toxins, proteins, hormones, vitamins and amino acids for diseases control (Fatope et al, 2000). Endophytes are synergistic to their host; among them some are thought to be making returns for the nutrition from the plant by producing special substances, such as secondary metabolites. It has been found that some Streptomyces have taken up residence in plants which opens the possibility that, this may be an entirely prime source of novel pharmaceuticals (Joseph and Priya, 2011)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
