Abstract
ABSTRACTSeveral studies have demonstrated strong antioxidant activity of date fruits. Nevertheless, limited data is available for the bioactive compounds responsible for the activity. In this study, the antioxidant activity of five different date varieties (Ajwa, Anbara, Piyarom, Rabbi and Deglet Nour) extracted using 80% ethanol was determined using free radical scavenging (DPPH), ferric reducing power (FRAP) and antioxidant capacity (ABTS) assays. The 1H- NMR was employed to profile the bioactive metabolites present in the dates. The obtained results demonstrated that the Piyarom extract had the highest total phenolic content (355 mg GAE/100g DW), total flavonoid content (57.07 mg/100g DW) and exhibited good antioxidant activity with IC50 of 16.2 µg/mL, 26.38 mmol Fe (II)/g and 11.3 µg/mL for DPPH scavenging activity, FRAP antioxidant power and ABTS antioxidant capacity, respectively. The metabolites responsible for the variation between different date’s varieties were successfully identified using 1H-NMR–based metabolomics. The principal component analysis (PCA) and partial least squares (PLS) analysis depicted clear and distinct separations into three clusters representing the five date varieties. The metabolites responsible for the separation were identified as sucrose, betaine, fructose, ascorbic acid, glycine, and arginine. Correlation study from PLS biplot revealed that glucose, ascorbic acid, epicatechin, gallic acid, and citric acid are responsible for the antioxidant activity measured.
Highlights
Great number of diseases including autoimmune disease, cancer, diabetes, coronary heart disease, atherosclerosis, sclerosis, chronic inflammation, and cataracts are induced by free radicals that are reactive oxygen species such as hydrogen peroxide, hydroxyl radical and superoxide anion radical
The results showed that ABTS activity of Piyarom, Rabbi, Ajwa, Anbara, and Deglet Nour were measured at 11.3, 18.7, 16.4, 14.3, and 19.1 mmol TE/100 g dry weight, respectively
Profiling of metabolites indicated the presence of 17 bioactive compounds in the ethanolic extract of five selected samples with various concentrations
Summary
Great number of diseases including autoimmune disease, cancer, diabetes, coronary heart disease, atherosclerosis, sclerosis, chronic inflammation, and cataracts are induced by free radicals that are reactive oxygen species such as hydrogen peroxide, hydroxyl radical and superoxide anion radical. Date fruits (Phoenix dactylifera L.) are rich sources of invaluable energy and nutrients. They have been reported to have anti-mutagenic and antioxidant activities as well as aid in reducing the risk of cardiovascular diseases.[6] reports on the properties of different date varieties obtained from different origins are still limited. This is more obvious for the bioactive metabolites responsible for the antioxidant activity of dates.
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