Abstract
BackgroundPlants represent an intricate and innovative source for the discovery of novel therapeutic remedies for the management of various ailments. The current study has been aimed to validate the therapeutic potential of ethnomedicinally significant plant Atriplex lasiantha Boiss.MethodsThe polarity based extraction process was carried out using fourteen solvents to figure out best extraction solvent and bioactive fractions. Total phenolic-flavonoids contents were quantified colorimetrically and polyphenolics were measured using HPLC-DAD analysis. Moreover, the test samples were tested against several diseases targets following various assays including free radicals scavenging, antibacterial, antifungal, cytotoxic and antileishmanial assay.ResultsAmong the solvent fractions, maximum yield was obtained with methanol-water extract i.e., 11 ± 0.49%. Maximum quantity of gallic acid equivalent phenolic content and quercetin equivalent flavonoid content were quantified in methanol-ethyl acetate extract of A. lasiantha. Significant quantity of rutin i.e., 0.3 μg/mg was quantified by HPLC analysis. The methanol-ethyl acetate extract of A. lasiantha exhibited maximum total antioxidant and total reducing power with 64.8 ± 1.16 AAE/mg extract respectively, while showing 59.8 ± 1.07% free radical scavenging potential. A significant antibacterial potential was exhibited by acetone-distilled water extract of A. lasiantha with 11 ± 0.65 mm zone of inhibition against B. subtilis. Considerable antifungal activity was exhibited by ethyl acetate-n-hexane extract of aerial part of A. lasiantha with 14 ± 1.94 mm zone of inhibition against A. fumigatus. Highest percentage of α-amylase inhibition (41.8 ± 1.09%) was observed in ethyl acetate-n-hexane extract. Methanol-acetone extract of A.lasiantha demonstrated significant inhibition of hyphae formation with 11 ± 0.49 mm bald zone of inhibition. Significant in-vitro cytotoxicity against Hep G2 cell line has been exhibited by methanol-chloroforms extract of A.lasiantha.ConclusionThe current study reveals the prospective potential of Atriplex lasiantha Boiss. for the discovery of biologically active compounds through bioassay guided isolation against various diseases.
Highlights
Plants represent an intricate and innovative source for the discovery of novel therapeutic remedies for the management of various ailments
Quantitative phytochemical screening Total phenolic and flavonoid content The profile of total phenolic and flavonoid content was determined from standard curve calibration of gallic acid and quercetin respectively
The phenolic and flavonoid chemical entities present widespread in plant kingdom with potential antioxidant nature
Summary
Plants represent an intricate and innovative source for the discovery of novel therapeutic remedies for the management of various ailments. The genus Atriplex encompassing about 300 species of angiosperms is well recognized ethnomedicinal genus which exists widely and utilized by aboriginal folks [12, 13] It belongs to family Chenopodiaceae which grows in Australia, Africa, Asia, and North America. These species are known as saltbushes, because they are halophytes in nature and have adapted to grow in dry environment with salty soil Due to their presence in extreme saline condition, a number of species of this group have been researched upon for their pharmacological activities, for example, secondary metabolites isolated from Atriplex nummularia and Atriples leucoclada possess anti ulcerative, anticolitis, antifungal and mollusicidal activity [14, 15]. In Pakistan, the leaves of Atriplex crossifolia are used in the treatment of yellow jaundice and throat infection [22]
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