Abstract
Common spruce (Picea abies L.) is a fast-growing coniferous tree, widely used in several countries for the production of sawn wood, timber and pulp. During this industrial exploitation, large quantities of barks are generated as waste materials. The aim of this study was the bio-guided investigation and the effective recovery of methanol-soluble metabolites of common spruce bark for the development of new dermo-cosmetic agents. The active methanol extract was initially fractionated by Centrifugal Partition Chromatography (CPC) using a triphasic solvent system in a step-gradient elution mode. All resulting fractions were evaluated for their antibacterial activity, antioxidant activity and their capability to inhibit tyrosinase, elastase and collagenase activity. In parallel, the chemical composition of each fraction was established by combining a 13C-NMR dereplication approach and 2D-NMR analyses. As a result, fourteen secondary metabolites corresponding to stilbene, flavonoid and phenolic acid derivatives were directly identified in the CPC fractions. A high amount (0.93 g) of E-astringin was recovered from 3 g of crude extract in a single 125 min run. E-Astringin significantly induced the tyrosinase activity while E-piceid, taxifolin, and taxifolin-3′-O-glucopyranoside exhibited significant anti-tyrosinase activity. The above compounds showed important anti-collagenase and antimicrobial activities, thus providing new perspectives for potential applications as cosmetic ingredients.
Highlights
IntroductionPicea abies (L.) Karst, (common spruce or Norway spruce) is a large evergreen coniferous species native to Northern, Central and Eastern Europe
Picea abies (L.) Karst, is a large evergreen coniferous species native to Northern, Central and Eastern Europe
In a single 125 min run, 3 g of the active methanol extract were fractionated by Centrifugal Partition Chromatography (CPC) using a triphasic solvent system in a step-gradient elution mode
Summary
Picea abies (L.) Karst, (common spruce or Norway spruce) is a large evergreen coniferous species native to Northern, Central and Eastern Europe. This fast-growing tree plays an important economical role in several countries where its wood is widely used for the production of sawn timber and pulp. In addition to plant disease resistance, these compounds have numerous biological activities that could find applications in human health. This large amount of bark wastes represents an interesting source of high-value chemicals giving added value to this energy resource [3]
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